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wiki2016_rna_clustering [2017/12/05 09:21]
erin
wiki2016_rna_clustering [2017/12/05 09:35] (current)
erin
Line 43: Line 43:
   * **Center & Scale** the reads    * **Center & Scale** the reads 
       * for each gene, set the mean expression across the dataset to 0; set the standard deviation to 1.       * for each gene, set the mean expression across the dataset to 0; set the standard deviation to 1.
-      * Use t(scale(matrix)) prior to calling the clustering function+      * Use t(scale(t(matrix))) prior to calling the clustering function
       * OR, look for a 'scale by rows' option within the clustering function       * OR, look for a 'scale by rows' option within the clustering function
       * Why? To focus in on the **trend** of each gene's expression pattern, not the intensity       * Why? To focus in on the **trend** of each gene's expression pattern, not the intensity
wiki2016_rna_clustering.txt ยท Last modified: 2017/12/05 09:35 by erin